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E-GEOD-35245 - Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells (methylation data)

Status
Released on 1 December 2012, last updated on 2 June 2014
Organism
Homo sapiens
Samples (22)
Array (1)
Protocols (8)
Description
We have investigated the effects of genistein on several prostate cancer cell lines, including the ARCaP-E/ARCaP-M model of the epithelial-to-mesenchymal transition (EMT), to analyze effects on their epigenetic state. In addition, we investigated the effects of combined treatment of genistein with the histone deacetylase inhibitor vorinostat on survival in prostate cancer cells. Using whole-genome expression profiling and whole-genome methylation profiling, we have determined the genome-wide differences in genetic and epigenetic responses to genistein in prostate cancer cells before and after undergoing the EMT. Also, cells were treated with genistein, vorinostat, and a combination treatment, where cell death and cell proliferation was determined. ARCAP-E, ARCAP-M, and normal human PrEC cells were analyzed for genome-wide methylation using the Illumina 27K CpG Methylation BeadChip. ARCAP-E and ARCAP-M cells were treated with DMSO as a negative control, genistein, or 5-aza-deoxycytidine as a positive control for demethylation. PrEC cells, used as a normal human prostate cell line control, were untreated.
Experiment type
methylation profiling by array 
Contacts
Khanjan Gandhi <khanjan.gandhi@emory.edu>, Carlos S Moreno, Christopher K Giardina, Cornel J Phillip, Omer Kucuk, Yu H Lai
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-35245.idf.txt
Sample and data relationshipE-GEOD-35245.sdrf.txt
Processed data (1)E-GEOD-35245.processed.1.zip
Additional data (1)E-GEOD-35245.additional.1.zip
Array designA-GEOD-8490.adf.txt
Links