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E-GEOD-3485 - RNAi knock down of LEDGF transcript from the PSIP1 gene in human 293T cells reveals a role for LEDFG/p75 in targeting HIV integration

Status
Released on 27 October 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Description
The LEDGF transcript from the PSIP1 gene was knocked down using RNAi technology. The resulting 293T derived cell line (293TsiLL) was compared to a control cell line (293TsiScram) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Experiment Overall Design: 2 cell lines were used, 293TsiLL (experimental) and 293TsiScram (control). 4 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.
Experiment types
RNAi profiling by array, cellular modification
Contact
Chris Hoffmann
Citation
A role for LEDGF/p75 in targeting HIV DNA integration. Angela Ciuffi, Manuel Llano, Eric Poeschla, Christian Hoffmann, Jeremy Leipzig, Paul Shinn, Joseph R Ecker, Frederic Bushman. Nat Med 11(12):1287-9 (2005)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-3485.idf.txt
Sample and data relationshipE-GEOD-3485.sdrf.txt
Processed data (1)E-GEOD-3485.processed.1.zip
Array designA-AFFY-44.adf.txt
Links