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E-GEOD-34667 - Transcription profiling by array of Arabidopsis G-protein knockout plants in response to ozone

Status
Released on 24 December 2011, last updated on 25 November 2015
Organism
Arabidopsis thaliana
Samples (27)
Array (1)
Protocols (8)
Description
Ozone pollution decreases plant growth and yield worldwide. Some of the effects are genetically-mediated and are reported to involve G-protein signaling pathways. Effects of ozone on gene expression were examined in wild-type and G-protein null mutants to determine affected genes and to determine differential responses that may help define affected pathways. We used microarrays to examine changes in gene expression in response to ozone exposure and identified distinct classes of up- and down-regulated genes in wild-type and G-protein null mutant genotypes. Columbia wild-type and G-protein null mutant gpa1-4/agb1-2 plants were exposed to 5 or 125 ppb ozone in controlled environment chambers for two days. Leaves were sampled after 3 h and 2 d of exposure to examine early and late gene expression changes.
Experiment type
transcription profiling by array 
Contacts
Fitzgerald Lewis Booker <fitz.booker@ars.usda.gov>, Alan Jones, Fitzgerald L Booker, Kent O Burkey
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-34667.idf.txt
Sample and data relationshipE-GEOD-34667.sdrf.txt
Raw data (1)E-GEOD-34667.raw.1.zip
Processed data (1)E-GEOD-34667.processed.1.zip
Array designA-AFFY-2.adf.txt
Links