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E-GEOD-34242 - miR-34a targets PDGFRA in proneural malignant glioma

Status
Released on 26 March 2012, last updated on 1 April 2012
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
To identify potential targets of miR-34a, we performed transcriptional profiling on proneural TS543 GBM cells, focusing on mRNAs whose levels decreased in response to miR-34a transfection as compared to control oligonucleotide. Proneural TS543 GBM cells were transfected with 100 nM hsa-miR-34a or control oligonucleotide using Hiperfect transfection reagent (Qiagen). After 3 days, RNA was isolated and expression analyses were performed using Illumina HT-12 bead array. The microarray dataset was normalized using a variance stable normalization (VSN) procedure in the ‘lumi’ package from the Bioconductor framework.
Experiment type
transcription profiling by array 
Contacts
Joachim Silber <geo@ncbi.nlm.nih.gov>, Alicia Pedraza, Anders Jacobsen, Chris Sander, Eric C Holland, Girish Harinath, Jason T Huse, Tatsuya Ozawa
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-34242.idf.txt
Sample and data relationshipE-GEOD-34242.sdrf.txt
Processed data (1)E-GEOD-34242.processed.1.zip
Array designA-MEXP-1171.adf.txt
Links