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E-GEOD-32600 - Distal airway stem cells yield alveoli in vitro and during lung regeneration following H1N1 influenza infection (colony)

Status
Released on 1 November 2011, last updated on 18 April 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
The extent of lung regeneration following catastrophic damage and the potential role of adult stem cells in such a process remains obscure. Sublethal infection of mice with an H1N1 influenza virus related to that of the 1918 pandemic triggers massive airway damage followed by apparent regeneration. We show here that p63-expressing stem cells in the bronchiolar epithelium undergo rapid proliferation after infection and radiate to interbronchiolar regions of alveolar ablation. Once there, these cells assemble into discrete, Krt5+ pods and initiate expression of markers typical of alveoli. Gene expression profiles of these pods suggest that they are intermediates in the reconstitution of the alveolar-capillary network eradicated by viral infection. The dynamics of this p63-expressing stem cell in lung regeneration mirrors our parallel findings that defined pedigrees of human distal airway stem cells assemble alveoli-like structures in vitro and suggests new therapeutic avenues to acute and chronic airway disease. 3 colonies fron control and H1N1 influenza treated lungs (12 dpi) ,each were picked for whole genome microarray analysis
Experiment type
transcription profiling by array 
Contacts
Frank Daniel McKeon <mckeonf@gis.a-star.edu.sg>, Christopher P Crum, Dakai Mu, De Y Wang, Elisabeth Zielonka, Frank McKeon, Lim S Joo, Neo B Hoe, Pooja A Kumar, Rania Daghers, Tay S Wei, Vincent T Chow, Wa Xian, Yan Sun, Yuanyu Hu, Yusuke Yamamoto
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32600.idf.txt
Sample and data relationshipE-GEOD-32600.sdrf.txt
Raw data (1)E-GEOD-32600.raw.1.zip
Processed data (1)E-GEOD-32600.processed.1.zip
Array designA-AFFY-98.adf.txt
R ExpressionSetE-GEOD-32600.eSet.r
Links