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E-GEOD-31440 - Expression data from RKO cells transfected with SPARCL1 and vector control

Status
Released on 29 September 2011, last updated on 12 October 2011
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (8)
Description
Colon cancer cell line RKO grown on Matrigel form a luminal-like structure after the transfection of SPARCL1 gene, which may imply the differentiation of the cells. To indentify genes regulated by SPARCL1, we built two stable cell lines: RKO with pLXSN-SPARCL1 plasmid (RKO-SPARCL1) and RKO with pLXSN plasmid (RKO-pLXSN). Then we cultured these two cell lines on both plastic dish and dish cover with a layer of Matrigel. We used microarrays to undentify global gene expression change in RKO cells after the tranfection of SPARCL1. Four samples including RKO-pLXSN cells cultured on plastic, RKO-pLXSN cells cultured on Matrigel, RKO-SPARCL1 cells cultured on plastic and RKO-SPARCL1 cells cultured on Matrigel were trypsinized and collected for RNA extraction and hybridization on Affymetrix microarray U133plus2.0. Supplementary files: * RLvsRP: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on plastic * RLMvsRPM: up and down regulated genes comparing with RKO-SPARCL1(RL) and RKO-pLXSN(RP) cells cultured on Matrigel
Experiment type
transcription profiling by array 
Contacts
Hang Zhang <bonnieele@gmail.com>, Hanguang Hu, Weiting Ge
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31440.idf.txt
Sample and data relationshipE-GEOD-31440.sdrf.txt
Raw data (1)E-GEOD-31440.raw.1.zip
Processed data (1)E-GEOD-31440.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-31440.eSet.r
Links