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E-GEOD-30807 - Expression data from human mesenchymal stem cell and osteosarcoma cells

Status
Released on 30 December 2012, last updated on 2 June 2014
Organism
Homo sapiens
Samples (3)
Array (1)
Protocols (8)
Description
To determine whether enhanced self-renewal and tumorigenicity in UT2 cells (derived from the second human–mouse xenotransplantation of U2OS cell-formed osteosarcoma tissues) correlate with increased expression of stem/progenitor cell-associated genes, we measured global gene expression in MSC, U2OS and UT2 cells by microarray analysis. Compared to U2OS and MSC, molecules involved in regulation of self-renewal signaling pathways of cancer stem cells were also up-regulated in UT2 cells, including those in the Notch, Wnt, and TGF-beta pathways. These data suggest a genetic basis for the enhanced self-renewal and tumorigenicity of osteosarcoma-initiating cell in UT2 cells. MSC, U2OS and UT2 cells were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to analysis stem/progenitor cell-associated genes and molecules involved in regulation of self-renewal signaling pathways of cancer stem cells between UT2 cells and its parent cells: U2OS (MSC works as positive control here).
Experiment type
transcription profiling by array 
Contacts
Lingtao Wu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30807.idf.txt
Sample and data relationshipE-GEOD-30807.sdrf.txt
Raw data (1)E-GEOD-30807.raw.1.zip
Processed data (1)E-GEOD-30807.processed.1.zip
Array designA-AFFY-44.adf.txt
Links