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E-GEOD-30431 - Whole mouse genome analysis of LCMV-specific CD4 and CD8 T cells throughout acute and chronic LCMV infection

Status
Released on 12 December 2012, last updated on 14 January 2013
Organism
Mus musculus
Samples (71)
Array (1)
Protocols (7)
Description
CD4 and CD8 T cells display functional defects during chronic infection such as loss of certain cytokines. Recent studies have suggested that CD4 T cells may actually gain other functions, however. Here, we analyzed gene expression profiles from LCMV-specific CD4 and CD8 T cells throughout the response to either acute LCMV or chronic LCMV infection. This alllowed us to identify CD4-specific changes during chronic infection compared to acute infection but also revealed shared core regulators between CD4 and CD8 T cells. LCMV-specific CD4 and CD8 T cells were isolated 6, 8, 15 and 30 days post infection with LCMV Armstrong or LCMV clone 13. Naïve CD4 and CD8 T cells were also isolated from naïve mice as comparisons. Four replicates of each sample were hybridized. The only exception is LCMV-specific CD4 T cells isolated 6 days post infection with LCMV-Arm where only three replicates were hybridized.
Experiment type
transcription profiling by array 
Contacts
Alison Crawford, E J Wherry, Jill M Angelosanto
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30431.idf.txt
Sample and data relationshipE-GEOD-30431.sdrf.txt
Raw data (2)E-GEOD-30431.raw.1.zip, E-GEOD-30431.raw.2.zip
Processed data (1)E-GEOD-30431.processed.1.zip
Array designA-AFFY-130.adf.txt
Links