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E-GEOD-29744 - Translational control in early adipogenesis

Status
Released on 28 March 2012, last updated on 26 June 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (8)
Description
In obesity, adipose tissue expands by increasing the volume of existing adipocytes (hypertrophy), increasing the number of small adipocytes (hyperplasia) or both. Much is known about transcriptional control during adipogenesis, including early events. However, translational control plays a pivotal role in many dynamic processes and we presume that it is also important for the control of adipogenesis, especially in the first hours after hormonal induction. By use of ribosome profiling we identified 43 genes that are up-regulated and 2 genes that are down-regulated during the first six hours of adipogenesis in 3T3-L1 cells. Interestingly, we found Ghrelin to be down-regulated. Up-regulated genes comprise factors that are nucleic acid binding (Cdkn1c, eIF4b, Hsf1, Irf6, Myc, Plekhn1, Polr2a, Rpl18, Rpl27a, Rpl6, Rpl7a, Rps18, Rpsa, Sema3g, Tbc1d22a, Tsc22d3), form part of ribosomes (L6, L7a, L18, L27a, Sa, S18, S15a pseudogene), act on the regulation of translation (eIF4b) or transcription (Hsf1, Irf6, Myc, Tsc22d3). Others act as chaperones (Bag3, Hspa8/Hsc70, Hsp90ab1) or in other metabolic or signals transducing processes. We conclude that a moderate reorganisation of the functionality of the ribosomal machinery is a very important step for the growth and expression control at the beginning of adipogenesis. 12 samples: 4 (0h, non-polysomal fraction; 0h, polysomal fraction; 6h, non-polysomal fraction; 6h, polysomal fraction) x3 replicates
Experiment type
transcription profiling by array 
Contacts
Silvia von der Heyde <silvia.heyde@med.uni-goettingen.de>, Bernhard G Baumgartner, Bertram Brenig, Carolin Fromm-Dornieden, Lennart Opitz, Olexandr Lytovchenko, Tim Beissbarth
Citation
Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. Fromm-Dornieden C, von der Heyde S, Lytovchenko O, Salinas-Riester G, Brenig B, Beissbarth T, Baumgartner BG. , PMID:22436005
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-29744.idf.txt
Sample and data relationshipE-GEOD-29744.sdrf.txt
Raw data (1)E-GEOD-29744.raw.1.zip
Processed data (1)E-GEOD-29744.processed.1.zip
Array designA-MEXP-724.adf.txt
Links