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E-GEOD-29195 - Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)

Status
Released on 7 October 2011, last updated on 2 May 2014
Organism
Homo sapiens
Samples (29)
Protocols (13)
Description
Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human ChIP-seq portion of this dataset on leukemia cell lines. Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008), CEBPA (SC-9314) or H3K4me1 (Abcam ab8895).
Experiment type
ChIP-seq 
Contacts
Teresa Venezia Bowman <geo@ncbi.nlm.nih.gov>, A DiBiase, A K Sessa, C Mosimann, C S Martin, D Wu, E M Durand, E Trompouki, G C Heffner, G Q Daley, J L Leblanc, J N Cech, L I Zon, L N Lawton, P Li, R A Young, R F Paulson, T V Bowman, Z P Fan
Citation
Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. Trompouki E, Bowman TV, Lawton LN, Fan ZP, Wu DC, DiBiase A, Martin CS, Cech JN, Sessa AK, Leblanc JL, Li P, Durand EM, Mosimann C, Heffner GC, Daley GQ, Paulson RF, Young RA, Zon LI. , PMID:22036566
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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