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E-GEOD-29187 - Transcription profiling by array of Arabidopsis overexpressing RAP2.12 or with RAP2.12 and RAP2.2 silenced after growth in hypoxic conditions

Status
Released on 13 October 2011, last updated on 30 April 2015
Organism
Arabidopsis thaliana
Samples (18)
Array (1)
Protocols (9)
Description
In this study we analyzed the effect of overexpression of an HA-tagged version of the ERF RAP2.12 on the transcriptome levels in aerobic and hypoxic-treated (O2 21% and 1%, respectively) Arabidopsis thaliana rosettes. We also analyzed the effect of a RAP2.12 and RAP2.2 simultaneous silencing in aerobic and hypoxic-treated (O2 21% and 1%, respectively) Arabidopsis thaliana rosettes. We treated Arabidopsis Col-0 (wt) rosettes and transgenic HA::RAP2.12 and amiRAP2.2-12 , 5-week old, grown in 8/16 light/dark photoperiod with: -Control (22°C, dark, 21% O2, 1.5h). -Hypoxia (22°C, dark, 1% O2, 1.5h).
Experiment type
transcription profiling by array 
Contacts
Francesco Licausi <f.licausi@sssup.it>, Federico M Giorgi, Joost T van Dongen
Citation
Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization. Licausi F, Kosmacz M, Weits DA, Giuntoli B, Giorgi FM, Voesenek LA, Perata P, van Dongen JT.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-29187.idf.txt
Sample and data relationshipE-GEOD-29187.sdrf.txt
Raw data (1)E-GEOD-29187.raw.1.zip
Processed data (1)E-GEOD-29187.processed.1.zip
Array designA-AFFY-2.adf.txt
Links