Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-28484 - Microarray analysis of chimeric CD4+CD28null and CD4+CD28+ T-cells in humanized mice

Status
Released on 5 January 2015, last updated on 10 January 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (6)
Description
The major goal of this experiment was to identify the overall changes in gene expression of human CD4+CD28 null T-cells that develop after repetitive [xeno] antigen stimulation, in comparison to normal CD4+CD28+ T-cells. These cells were derived by adoptive transfer of human CD4 T-cells from a normal donor (all CD28+) into immunodeficient mice. Chimeric human CD4+CD28+ were isolated from 4 mice, and chimeric human CD4+CD28 null cells from two of these animals after expansion and T-cell differentiation in vivo. Animals were euthanized, and single cell suspensions of splenocytes derived. CD4 T-cell subpopulations were isolated by fluoresence activated cell sorting, RNA was extrated, labeled and hybridized to whole human gene expression arrays. Overall changes in the gene expression were identified using GEDI (gene expression dynamic inspector). 603 genes were found to be statistically significant (P<0.05) between the CD4+CD28+ and CD4+CD28null cells. Candidate genes were validated using qRT-PCR
Experiment type
transcription profiling by array 
Contacts
JOSE DAVID HERAZO MAYA <geo@ncbi.nlm.nih.gov>, Jianmin Xue, Jose Herazo-Maya, Steve Duncan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-28484.idf.txt
Sample and data relationshipE-GEOD-28484.sdrf.txt
Raw data (1)E-GEOD-28484.raw.1.zip
Processed data (1)E-GEOD-28484.processed.1.zip
Array designA-AGIL-28.adf.txt
Links