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E-GEOD-27674 - Protoanemonin: A new natural quorum sensing inhibitor that selectively activates iron starvation response

Released on 12 June 2012, last updated on 21 June 2012
Pseudomonas aeruginosa
Samples (4)
Array (1)
Protocols (8)
Many Gram-negative bacteria employ cell-to-cell communication mediated by N-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. Outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. We here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads Pseudomonas sp. B13 and Pseudomonas reinekei MT1 as a blind end in the biodegradation of organochloride xenobiotics, which inhibits quorum sensing in P. aeruginosa in naturally occurring concentrations. This catabolite, 4-methylenebut-2-en-4-olide, also known as protoanemonin, has been reported to possess antibacterial properties, but seems to have dual functions. Using transcriptomics and proteomics, we found that protoanemonin significantly reduced expression of genes and secretion of proteins known to be under control of quorum sensing in P. aeruginosa. Moreover, we found activation of genes and gene products involved in iron starvation response. It is thus likely that inhibition of quorum sensing, as the production of antibiotics, is a phenomenon found in complex bacterial communities. Strain P. aeruginosa MPAO1 was cultivated under defined ABT medium. The orginal culture was divided in the early exponential phase into two parts. One was treated with 125μM Protoanemonin and the other served as a control. The experiment was performed in duplicates.
Experiment type
transcription profiling by array 
Piotr Bielecki <>, Jacek Puchałka, Mette E Skindersoe, Michael Givskov, Roberto A Bobadilla Fazzini, Vitor A P Martins dos Santos
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response. Bobadilla Fazzini RA, Skindersoe ME, Bielecki P, Puchałka J, Givskov M, Martins Dos Santos VA. , PMID:22672701
Investigation descriptionE-GEOD-27674.idf.txt
Sample and data relationshipE-GEOD-27674.sdrf.txt
Raw data (1)
Processed data (1)
Array designA-AFFY-30.adf.txt
R ExpressionSetE-GEOD-27674.eSet.r