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E-GEOD-26148 - Expression profiling of MCF10A cells in 2D and 3D culture

Status
Released on 1 June 2011, last updated on 27 March 2012
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (7)
Description
In these microarray experiments, we characterize the gene expression of mammary epithelial cells (MCF10A cells) grown in either a traditional monolayer cell culture setting (2D) or on Matrigel, which induces single MCF10A cells to form organized acinar structures (3D). Morphogenesis of mammary epithelial cells into organized acinar structures in vitro is accompanied by widespread changes in gene expression patterns, including a substantial decrease in expression of Myc. The purpose of this study was to analyze the impact of morphogenesis and organization on gene expression with respect to changes in overall gene expression and Myc target gene expression. MCF10A cells were cultured in 2D for either 2 or 5 days (3 biological replicates each) or in 3D for 8 or 16 days (3 or 5 biological replicates, respectively)
Experiment type
transcription profiling by array 
Contacts
David Simpson <geo@ncbi.nlm.nih.gov>, David R Simpson, Min Yu, Senthil K Muthuswamy, Siyuan Zheng, William P Tansey, Zhongming Zhao
Citation
Epithelial Cell Organization Suppresses Myc Function by Attenuating Myc Expression. Simpson DR, Yu M, Zheng S, Zhao Z, Muthuswamy SK, Tansey WP. , PMID:21610111
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26148.idf.txt
Sample and data relationshipE-GEOD-26148.sdrf.txt
Raw data (1)E-GEOD-26148.raw.1.zip
Processed data (1)E-GEOD-26148.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-26148.eSet.r
Links