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E-GEOD-25095 - Protective Role of IL-10 in Ozone-induced Pulmonary Inflammation

Status
Released on 18 October 2011, last updated on 5 December 2011
Organism
Mus musculus
Samples (26)
Array (1)
Protocols (8)
Description
Background: The mechanisms underlying ozone (O3)-induced pulmonary inflammation remain unclear. Interleukin (IL)-10 is an anti-inflammatory cytokine that is known to inhibit inflammatory mediators. Objectives: The current study investigated the molecular mechanisms underlying IL-10-mediated attenuation of O3-induced pulmonary inflammation in mice. Methods: Il10-deficient (Il10-/-) and wild type (Il10+/+) mice were exposed to 0.3-ppm O3 or filtered air for 24, 48 or 72 hr. Immediately following exposure, differential cell counts, and total protein (a marker of lung permeability) were assessed from bronchoalveolar lavage fluid (BALF). mRNA and protein levels of cellular mediators were determined from lung homogenates. We also utilized global mRNA expression analyses of lung tissue with Ingenuity Pathway Analyses (IPA) to identify patterns of gene expression through which IL-10 modifies O3-induced inflammation. Results: Mean numbers of BALF polymorphonuclear leukocytes (PMNs) were significantly greater in Il10-/- mice than in Il10+/+ mice after exposure to O3 at all time points tested. O3-enhanced nuclear NF-kB translocation was elevated in the lungs of Il10-/- compared to Il10+/+ mice. Gene expression analyses revealed several key IL-10 and O3-dependent mediators, including IL-6, MIP-2, IL-1 and CD86. Conclusions: Results indicated that IL-10 protects against O3-induced pulmonary neutrophilic inflammation and cell proliferation. Moreover, gene expression analyses identified three response pathways and several novel genetic targets (e.g. Ccr1, Socs3, Il33, Hat1, and Gale) through which IL10 may modulate the innate and adaptive immune response. These novel mechanisms of protection against the pathogenesis of O3-induced pulmonary inflammation may also provide potential therapeutic targets to protect susceptible individuals. PARALLEL study design with 26 samples. Biological replicates: 2 to 3 replicates per group with wild type air exposed animals as controls for each time point (24, 48, 72 hours). Time-Course, Dose-Response, Strain comparison
Experiment type
transcription profiling by array 
Contacts
Steven R Kleeberger <kleeber1@niehs.nih.gov>, A K Bauer, D B Peden, Gillian S Backus, Hye-Youn Cho, Jacqueline Marzec, Jennifer Fostel, R Howden, Steve R Kleeberger
Citation
Protective role of interleukin-10 in ozone-induced pulmonary inflammation. Backus GS, Howden R, Fostel J, Bauer AK, Cho HY, Marzec J, Peden DB, Kleeberger SR. , PMID:20826374
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25095.idf.txt
Sample and data relationshipE-GEOD-25095.sdrf.txt
Raw data (1)E-GEOD-25095.raw.1.zip
Processed data (1)E-GEOD-25095.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-25095.eSet.r
Links