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E-GEOD-24291 - Expression data from differentiating ES cells expressing Snail during Wnt inhibition

Status
Released on 21 September 2011, last updated on 12 October 2011
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a global picture of ES cell differentiation at early timepoints after Snail was expressed during Wnt inhibition. A2.snail ES cells, which express Snail upon addition of doxycycline, were differentiated as embryoid bodies in differentiation media and DKK1. Total RNA was harvested from control (no doxycycline) and Snail-induced (doxycycline at day 2) cultures at 6, 12, and 24 hours after doxycycline addition (corresponding to days 2.25, 2.5, and 3 of differentiation).
Experiment type
transcription profiling by array 
Contacts
Jennifer Gill <geo@ncbi.nlm.nih.gov>, Ellen M Langer, Jennifer G Gill, Kenneth M Murphy, Mi Cai, Michael Kyba, Robert C Lindsley, Theresa L Murphy
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-24291.idf.txt
Sample and data relationshipE-GEOD-24291.sdrf.txt
Raw data (1)E-GEOD-24291.raw.1.zip
Processed data (1)E-GEOD-24291.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-24291.eSet.r
Links