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E-GEOD-23212 - Gene expression profiling of mouse splenic Dendritic cells subsets

Status
Released on 28 July 2010, last updated on 27 March 2012
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (7)
Description
We describe a novel subset of CD8+ DCs in lymphoid organs of naïve mice characterized by expression of the CX3CR1 chemokine receptor. CX3CR1+CD8+ DCs lack hallmarks of classical CD8+ DCs, including IL12 secretion, the capacity to cross-present antigen and their developmental independence of the transcriptional factor BatF3. Gene expression profiling showed that CX3CR1+CD8+ DCs resemble CD8- cDCs. The microarray analysis further revealed a unique plasmacytoid DC (PDC) gene signature of CX3CR1+ CD8+ DCs. A PDC relationship of the cells is further supported by the fact that they harbor characteristic D-J immunoglobulin gene rearrangements and that development of CX3CR1+CD8+ DCs requires E2-2, the critical transcriptional regulator of PDCs. Thus, CX3CR1+ CD8+ DCs represent a unique DC subset, related to but distinct from PDCs. After collagenase D digestion, spleens from Cx3cr1gfp/+ C57BL/6 mice were enriched for CD11c+ cells by magnetic separation according to the manufacturer’s protocol (MiltenyiBiotec GmbH). Splenic CD11chi cells were isolated using the FACS ARIA high-speed sorter (Becton-Dickinson). Total RNA was extracted and subjected to gene expression profiling using the Mouse Genome 430.2 Affymetrix GeneChip
Experiment type
transcription profiling by array 
Contacts
Steffen Jung <s.jung@weizmann.ac.il>, Boris Reizis, Dunja Bruder, Jan Buer, Kanako L Lewis, Kenneth M Murphy, Liat Bar-On, Tal Birnberg
Citation
CX3CR1+ CD8alpha+ dendritic cells are a steady-state population related to plasmacytoid dendritic cells. Bar-On L, Birnberg T, Lewis KL, Edelson BT, Bruder D, Hildner K, Buer J, Murphy KM, Reizis B, Jung S. , PMID:20679228
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23212.idf.txt
Sample and data relationshipE-GEOD-23212.sdrf.txt
Raw data (1)E-GEOD-23212.raw.1.zip
Processed data (1)E-GEOD-23212.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-23212.eSet.r
Links