Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-22904 - Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains
Released on 17 December 2010, last updated on 1 May 2014
An S288c-derived lab strain of Saccharomyces cerevisiae has lower ethanol resistance than either the vineyard strain M22 or the oak strain YPS163. We performed a 60 minute time-course experiment in the presence of 5% ethanol and compared the transcriptional response to ethanol between strains. We additionally analyzed the time-point of maximal response (30 min.) in wild type cells, YPS163 msn2D, and YPS163 hap1D cells. The genomic expression response to 5% ethanol stress was measured in 3 different strain backgrounds, and 2 different mutant backgrounds. Basal gene expression was also compared in two non-lab strains to the S288c reference. A single replicate was performed for the time-course experiment. All of the single time-point experiments were performed using biological triplicates.
transcription profiling by array
Jeffrey A. Lewis <firstname.lastname@example.org>, Alan J Higbee, Audrey P Gasch, Isaac M Elkon, Jeffrey A Lewis, McSean A McGee
Exploiting natural variation in Saccharomyces cerevisiae to identify genes for increased ethanol resistance. Lewis JA, Elkon IM, McGee MA, Higbee AJ, Gasch AP. , PMID:20855568