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E-GEOD-22904 - Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains

Status
Released on 17 December 2010, last updated on 1 May 2014
Organism
Saccharomyces cerevisiae
Samples (72)
Array (1)
Protocols (9)
Description
An S288c-derived lab strain of Saccharomyces cerevisiae has lower ethanol resistance than either the vineyard strain M22 or the oak strain YPS163. We performed a 60 minute time-course experiment in the presence of 5% ethanol and compared the transcriptional response to ethanol between strains. We additionally analyzed the time-point of maximal response (30 min.) in wild type cells, YPS163 msn2D, and YPS163 hap1D cells. The genomic expression response to 5% ethanol stress was measured in 3 different strain backgrounds, and 2 different mutant backgrounds. Basal gene expression was also compared in two non-lab strains to the S288c reference. A single replicate was performed for the time-course experiment. All of the single time-point experiments were performed using biological triplicates.
Experiment type
transcription profiling by array 
Contacts
Jeffrey A. Lewis <jalewis4@wisc.edu>, Alan J Higbee, Audrey P Gasch, Isaac M Elkon, Jeffrey A Lewis, McSean A McGee
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22904.idf.txt
Sample and data relationshipE-GEOD-22904.sdrf.txt
Raw data (1)E-GEOD-22904.raw.1.zip
Processed data (1)E-GEOD-22904.processed.1.zip
Array designA-GEOD-5915.adf.txt
Links