E-GEOD-21837 - Expression data from unactivated vs. activated PBMCs
Released on 28 May 2010, last updated on 10 September 2015
Long-lasting activation of T cells requires up-regulation of many genes, for example of transcription factors, cytoskeletal proteins and cell surface proteins including ion channels. An increase of ion channel density at the cell surface reflects the needs to manage increased Ca2+ influx into the activated T cell. Using oligonucleotide-based arrays we have surveyed changes in ion channel mRNA expression that occur upon T cell activation. We used Affymetrix Analysis to confirm our data achieved by self-designed glass array analysis. We isolated PBMCs from blood of healthy donors and divided those into two parts. One was activated for 72 hr by adding PHA-L whereas the other part was incubated in the same way but not stimulated.
transcription profiling by array
Christoph Ogrodowczyk <email@example.com>, C Ogrodowczyk, O Pongs
A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. Abramowski P, Ogrodowczyk C, Martin R, Pongs O.