E-GEOD-21837 - Expression data from unactivated vs. activated PBMCs

Status
Released on 28 May 2010, last updated on 10 September 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
Long-lasting activation of T cells requires up-regulation of many genes, for example of transcription factors, cytoskeletal proteins and cell surface proteins including ion channels. An increase of ion channel density at the cell surface reflects the needs to manage increased Ca2+ influx into the activated T cell. Using oligonucleotide-based arrays we have surveyed changes in ion channel mRNA expression that occur upon T cell activation. We used Affymetrix Analysis to confirm our data achieved by self-designed glass array analysis. We isolated PBMCs from blood of healthy donors and divided those into two parts. One was activated for 72 hr by adding PHA-L whereas the other part was incubated in the same way but not stimulated.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-21837.idf.txt
Sample and data relationshipE-GEOD-21837.sdrf.txt
Raw data (1)E-GEOD-21837.raw.1.zip
Processed data (1)E-GEOD-21837.processed.1.zip
Array designA-AFFY-37.adf.txt
Links