E-GEOD-18982 - Transcription profiling by array of Arabidopsis grown at low pH

Status
Released on 17 November 2009, last updated on 30 April 2015
Organism
Arabidopsis thaliana
Samples (12)
Array (1)
Protocols (6)
Description
Plants live in soils that vary considerably, both spatially and over time, in terms of nutrient composition and pH. Consistently, plants have to recognize and adapt to these changes by altering their structure and metabolism. The goal of this array analysis is to characterize the global transcriptional response to external pH changes in roots, which to date is almost unexplored. Arabidopsis thaliana (Columbia-0) were grown in hydroponic cultures in basic nutrient solution. Two days before treatment the media was shifted to nutrient solution containing 5mM MES, pH 6. At the time of the treatment start (4 hours after light on) the plants were shifted to nutrient solutions of pH 4.5 and 6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes. Keywords: Expression profilling by array 12 samples were used in this experiment
Experiment types
transcription profiling by array, growth condition design, time series design
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18982.idf.txt
Sample and data relationshipE-GEOD-18982.sdrf.txt
Raw data (1)E-GEOD-18982.raw.1.zip
Processed data (1)E-GEOD-18982.processed.1.zip
Array designA-AFFY-2.adf.txt
Links