Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-18592 - Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells

Status
Submitted on 14 October 2009, released on 7 May 2010, last updated on 27 March 2012
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (9)
Description
Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes. MCF7 cells were grown in hormone depleted media for three days before a 1 hour treatment with E2 or 0.1 % ethanol (vehicle). Total RNA was collected using standard methods and polysome-association RNA from the same cells were collected using sucrose gradient fractionation. Both RNA populations were purified, labeled, and hybridized to Affymetrix Human Genest arrays.
Experiment type
transcription profiling by array 
Contacts
Alexander Brodsky <alex_brodsky@brown.edu>, Alexander S Brodsky, Sara Hillenmeyer
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18592.idf.txt
Sample and data relationshipE-GEOD-18592.sdrf.txt
Raw data (1)E-GEOD-18592.raw.1.zip
Processed data (1)E-GEOD-18592.processed.1.zip
Array designA-AFFY-141.adf.txt
R ExpressionSetE-GEOD-18592.eSet.r
Links