E-GEOD-17482 - Gene expression profile of human prostate cancer cells transfected with Stat3 siRNA or Stat5a/b siRNA
Released on 27 May 2010, last updated on 4 September 2015
Identification of the molecular changes that promote viability and metastatic behaviour of prostate cancer cells is critical for the development of improved therapeutic interventions for prostate cancer. Stat5a/b and Stat3 are both constitutively active in locally-confined and advanced prostate cancer, and both transcription factors have been reported to be critical for the viability and growth of prostate cancer cells. We used microarrays to compare gene expression profiles regulated by Stat5a/b vs. Stat3 in human prostate cancer cells. DU145 and CWR22Rv1 human prostate cancer cells were transfected with Stat3 siRNA, Stat5a/b siRNA or scramble siRNA as control. After 48 h, the cells were harvested and total RNA was prepared for Affymetrix microarrays.
transcription profiling by array
Marja Nevalainen <Marja.Nevalainen@jefferson.edu>, Abhijit Dasgupta, Ayush Dagvadorj, Benjamin Leiby, Gloria Bonucelli, Jacqueline Lutz, Johng Rhimm, Lei Gu, Lukas Bubendorf, Marja T Nevalainen, Michael P Lisanti, Paolo Fortina, Sankar Addya, Terry M Hyslop
Transcription factor Stat3 stimulates metastatic behavior of human prostate cancer cells in vivo, whereas Stat5b has a preferential role in the promotion of prostate cancer cell viability and tumor growth. Gu L, Dagvadorj A, Lutz J, Leiby B, Bonuccelli G, Lisanti MP, Addya S, Fortina P, Dasgupta A, Hyslop T, Bubendorf L, Nevalainen MT.