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E-GEOD-13496 - Transcription profiling of human aging hematopoietic progenitor/stem cells

Status
Submitted on 6 November 2008, released on 13 January 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (30)
Array (1)
Protocols (4)
Description
To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and mobilized stem cell products (34P38NPBSC) were examined using microarray-based expression profiling. Differential expression changes were confirmed by microarray comparisons of younger and older expanded T-cell populations. Experiment Overall Design: We compared gene expression profiles between younger and older human CD34+ cells from bone marrow (34BM) and CD34+CD38- cells from mobilized stem cell products(34P38NPBSC). We also examined gene expression changes associated with aging in human expanded T-cells. For 34BM and T-cells, biotin-labeling of total RNA (5 µg) was performed as per standard Affymetrix protocol. For the 34P38NPBSC arrays, the single-stranded linear amplification protocol was used to process total RNA (100 nanograms) for the microarrays. Both protocols are described in detail in PMID14706461.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Decreased IRF8 expression found in aging hematopoietic progenitor/stem cells. Choi Stirewalt, Pogosova-Agadjanyan Sharpless, Yukawa Cronk, Wood Larson, Radich Appelbaum, Heimfeld.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13496.idf.txt
Sample and data relationshipE-GEOD-13496.sdrf.txt
Raw data (1)E-GEOD-13496.raw.1.zip
Processed data (1)E-GEOD-13496.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-13496.eSet.r
Links