Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-13496 - Transcription profiling of human aging hematopoietic progenitor/stem cells
Submitted on 6 November 2008, released on 13 January 2009, last updated on 27 March 2012
To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and mobilized stem cell products (34P38NPBSC) were examined using microarray-based expression profiling. Differential expression changes were confirmed by microarray comparisons of younger and older expanded T-cell populations. Experiment Overall Design: We compared gene expression profiles between younger and older human CD34+ cells from bone marrow (34BM) and CD34+CD38- cells from mobilized stem cell products(34P38NPBSC). We also examined gene expression changes associated with aging in human expanded T-cells. For 34BM and T-cells, biotin-labeling of total RNA (5 µg) was performed as per standard Affymetrix protocol. For the 34P38NPBSC arrays, the single-stranded linear amplification protocol was used to process total RNA (100 nanograms) for the microarrays. Both protocols are described in detail in PMID14706461.
transcription profiling by array, unknown experiment type
Decreased IRF8 expression found in aging hematopoietic progenitor/stem cells. Choi Stirewalt, Pogosova-Agadjanyan Sharpless, Yukawa Cronk, Wood Larson, Radich Appelbaum, Heimfeld.