Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-13485 - Profiling 20,077 genes in 25 vaccine recipients: U133 Plus 2.0, Custom CDF Version 9

Status
Released on 22 November 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (87)
Array (1)
Protocols (6)
Description
The immune responses generated by YF-17D by profiling 20,077 genes in 25 vaccine recipients were accessed at days 1, 3, 7, and 21 post-vaccination compared to pre-vaccination in PBMCs. The immune responses generated by YF-17D by profiling 20,077 genes in 25 vaccine recipients were accessed at days 1, 3, 7, and 21 post-vaccination compared to pre-vaccination in PBMCs. Keywords: time course This study is a combination of 2 separate YF-17D vaccination trials. Trial 1 has 15 subjects, and Trial 2 has 10 subjects. The two trials occurred 1 year apart and used different lots of the vaccine. Enrolled volunteers were healthy, aged 18 to 45, and signed a written informed consent form. Potential volunteers were excluded from participating in the study if they were pregnant, or if they had been vaccinated previously with YF-17D. Blood samples were taken pre-vaccinaion to serve as a reference point for each individual, and post-vaccination samples were taken a days 1, 3, 7, and 21 after YF-17D vaccination. All subjects received YF-17D. Blood samples for microarray analysis were collected either in blue and black topped citrate buffered cell preparation tubes (CPT). Following PMBC isolation from CPT, 2 x 106 cells were lysed in 1 ml of TRIzol and stored at -80?C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.
Experiment type
transcription profiling by array 
Contacts
Troy D Querec, Aderem Alan, Ahmed Rafi, Akondy Rama, Bennouna Soumaya, Cao Weiping, Deng Jiusheng, Gernert Kim, Kennedy Kathleen, Lee Eva, Marzolf Bruz, Miller Joe, Mulligan Mark, Nakaya Helder, Olouch Herold, Pirani Ali, Pulendran Bali, Querec Troy, Teuwen Dirk, Vencio Ricardo, Wu Haiyan
Citation
Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans. Querec TD, Akondy RS, Lee EK, Cao W, Nakaya HI, Teuwen D, Pirani A, Gernert K, Deng J, Marzolf B, Kennedy K, Wu H, Bennouna S, Oluoch H, Miller J, Vencio RZ, Mulligan M, Aderem A, Ahmed R, Pulendran B.
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