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E-GEOD-13278 - Expression analysis to identify inducible genes in T cells

Status
Submitted on 19 October 2008, released on 9 April 2010, last updated on 27 March 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (8)
Description
Expression microarrays were employed to identify genes induced by phorbol ester and ionomycin stimulation of EL4 cells. EL4 is a murine T cell line. To identify induced genes that were independent of new protein synthesis cells were pre-treated with cycloheximide. This expression study was used in conjunction with histone acetylation ChIP-chip to determine if inducible genes had a specific histone acetylation profile and whether the acetylation profile differed for genes with different kinetics of induction. Keywords: drug treatment comparison Cells were pre-treated with either DMSO or cylcoheximide in DMSO, then they were stimulated with PMA and Ionomycin for either 0h or 4h. 3 biological replicates were used for each treatment-stimulation combination.
Experiment type
transcription profiling by array 
Contacts
Kristine Hardy <u4096741@anu.edu.au>, Chloe Lim, Frances Shannon, Kaimen Peng, Lina Ma
Citation
Defining the chromatin signature of inducible genes in T cells. Lim PS, Hardy K, Bunting KL, Ma L, Peng K, Chen X, Shannon MF.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13278.idf.txt
Sample and data relationshipE-GEOD-13278.sdrf.txt
Raw data (1)E-GEOD-13278.raw.1.zip
Processed data (1)E-GEOD-13278.processed.1.zip
Array designA-AFFY-130.adf.txt
R ExpressionSetE-GEOD-13278.eSet.r
Links