E-GEOD-13278 - Expression analysis to identify inducible genes in T cells
Submitted on 19 October 2008, released on 9 April 2010, last updated on 27 March 2012
Expression microarrays were employed to identify genes induced by phorbol ester and ionomycin stimulation of EL4 cells. EL4 is a murine T cell line. To identify induced genes that were independent of new protein synthesis cells were pre-treated with cycloheximide. This expression study was used in conjunction with histone acetylation ChIP-chip to determine if inducible genes had a specific histone acetylation profile and whether the acetylation profile differed for genes with different kinetics of induction. Keywords: drug treatment comparison Cells were pre-treated with either DMSO or cylcoheximide in DMSO, then they were stimulated with PMA and Ionomycin for either 0h or 4h. 3 biological replicates were used for each treatment-stimulation combination.
transcription profiling by array
Kristine Hardy <email@example.com>, Chloe Lim, Frances Shannon, Kaimen Peng, Lina Ma
Defining the chromatin signature of inducible genes in T cells. Lim PS, Hardy K, Bunting KL, Ma L, Peng K, Chen X, Shannon MF.