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E-GEOD-10173 - Appressorium induction by cyclic AMP in Magnaporthe grisea

Status
Submitted on 14 January 2008, released on 26 May 2010, last updated on 1 May 2014
Organism
Magnaporthe grisea
Samples (8)
Array (1)
Protocols (12)
Description
This series examines differential gene expression during appressorium formation induced by exogeous cyclic AMP in the rice blast fungus Magnaporthe grisea. RNA was extracted from spores germinated on hydrophilic (non-inductive) side of GelBond as well as from spores elaborating appressia on hydrophilic surfaces in the presence of cAMP after 9 hrs incubation. RNA from two biological replications of each treatment was pooled in equal amounts and labeled with both cy3 and cy5 dyes using the Agilent Technologies Low Input Linear Amplification Kit. Hybridizations were performed on Agilent Technologies whole genome oligo rice blast arrays (product G4137B) using manufacturer prescribed protocols and reagents in an reference design in which each treatment was paired with every other. This series contains a total of 4 hybridizations; each treatment was used in 4 hybridizations (2 with Cy3 and 2 Cy5). Spot fluorescence was normalized using Lowess within and between microarray slides in Bioconductor. Keywords: Cell development Two-condition experiment, cyclic AMP treated vs. non treated spores. 4 biological replicates with dye-swaps
Experiment type
unknown experiment type 
Contacts
Yeon Yee Oh <yoh2@ncsu.edu>, Huaqin Pan, Ralph A Dean, Sean Coughlan, Yeonyee Oh
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-10173.idf.txt
Sample and data relationshipE-GEOD-10173.sdrf.txt
Raw data (1)E-GEOD-10173.raw.1.zip
Processed data (1)E-GEOD-10173.processed.1.zip
Experiment designE-GEOD-10173.biosamples.png, E-GEOD-10173.biosamples.svg
Array designA-GEOD-2874.adf.txt
Links