E-CBIL-34 - Transcription profiling of rat insulinoma (INS-1 derived) cells grown in lipid culture
Submitted on 24 July 2007, released on 11 September 2007, last updated on 2 June 2014
This experiment was designed to study the effects of lipid culture on INS-1 derived glucose-responsive 832/13 cells. After 48-hr culture with lipids, a dramatic decrease in glucose-responsiveness is observed. In order to detect some earlier changes, RNA was also collected at earlier time points (12 hr and 24 hr). To compensate for any changes associated with cell density, control cells (1%BSA culture) were included for each time point. RNA was prepared from INS-1 cells in Dr. Chris Newgard's lab, quantified and sent frozen in water. Six biological replicates were sent for the following conditions: (i) 1%BSA (12, 24, and 48 hours) (ii) 1mM-Oleate/Palmitate (12, 24, and 48 hours).
transcription profiling by array, growth condition, reference, time series
Functional genomics of the beta-cell: SCHAD regulates insulin secretion independent of K+ currents. Hardy O.T., Hohmeier H.E., Becker T.C., Manduchi E., Doliba N.M., Gupta R.K., White P., Stoeckert C.J. Jr., Matschinsky F.M., Newgard C.B., Kaestner K.H. Mol Endocrinol (2006)