Bioinformatics: Introduction to Metabolomics
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Date:
Wednesday 20 May 2015Venue:
University of Cambridge - Downing Site, Cambridge, CB2 3AR, United KingdomParticipation:
First come, first servedContact:
Gabriella RusticiRegistration closed
Course Overview
The goal of metabolomics is to identify and quantify the complete biochemical composition of a biological sample. With the increase in genomic, transcriptomic and proteomic information there is a growing need to understand the metabolic phenotype that these these genes and proteins ultimately control. The aim of this course is to provide an overview of the applications, laboratory equipment and online bioinformatic portals for metabolomics research.
During the course we will introduce the common laboratory equipment in order to extract metabolites and analyse samples to collect metabolomic data (such as HPLC, GC and MS), how to analyse the data and identify the metabolites using online databases and how to map the metabolomic data in relation to metabolic pathways and the genes and proteins that are associated with those metabolic pathways. The course content will predominantly be based on analysing samples from model plant species such as Arabidopsis thaliana but the procedures are transferable to all other organisms.
Programme
| Time | Topic |
|---|---|
| Day 1 - Morning | |
| 09:30 - 10:00 | Introduction to Metabolomics |
| 10:00 - 11:00 | Overview of techniques |
| 11:00 - 11:20 | Coffee break |
| 11:20 - 12:30 | How to Identify a metabolite |
| 12:30 - 13:00 | Data standards initiatives |
| Day 1 -Afternoon | |
| 14:00 - 14:30 | Introduction to chemometrics |
| 14:30 - 15:30 | Online practical involving multivariate statistics |
| 15:30 - 15:50 | Tea break |
| 15:50 - 16:20 | Metabolic mapping |
| 16:20 - 17:00 | Online practical - Metabolic mapping |
| 17:00 - 17:30 | Further Questions and answers |
Additional information:
Additional Resources: Some URLs that provide online or downloadable databases and/or tools for identifying metabolites, their pathways and related genetic, transcriptomic and proteomic information.
- Metaboanalyst
- Plant Metabolic Network (PMN)
- Kanehisa Laboratories
- Kyoto Encyclopaedia of Genes and Genomes (KEGG) LIGAND database
- Chemical Entities of Biological Interest (ChEBI)
- Chemspider
- EcoCyc - Encyclopedia of Escherichia coli K-12 Genes and Metabolism
- Human Metabolome Project (HMP)
- KaPPA-View
- KNApSAck
- MetaCyc Encyclopaedia of Metabolic Pathways
- MetExplore
- Pathway Tools Omics Viewer
- Metabolome Japan