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PDBsum entry 6j0c
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Protein transport
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PDB id
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6j0c
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PDB id:
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| Name: |
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Protein transport
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Title:
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Cryo-em structure of an extracellular contractile injection system, pvc sheath complex in contracted state
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Structure:
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Pvc2. Chain: a, b, c, d, e, f, a, b, c, d, e, f. Engineered: yes
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Source:
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Photorhabdus asymbiotica subsp. Asymbiotica (strain atcc 43949 / 3105-77). Xenorhabdus luminescens (strain 2). Organism_taxid: 553480. Strain: atcc 43949 / 3105-77. Gene: pau_03352, pa-rva20-21-0170. Expressed in: escherichia coli. Expression_system_taxid: 562
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Authors:
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F.Jiang,N.Li,X.Wang,J.Cheng,Y.Huang,Y.Yang,J.Yang,B.Cai,Y.Wang,Q.Jin, N.Gao
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Key ref:
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F.Jiang
et al.
(2019).
Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.
Cell,
177,
370.
PubMed id:
DOI:
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Date:
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23-Dec-18
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Release date:
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10-Apr-19
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PROCHECK
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Headers
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References
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B6VNP3
(B6VNP3_PHOAA) -
Phage tail sheath protein from Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77)
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Seq:
Struc:
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355 a.a.
354 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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DOI no:
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Cell
177:370
(2019)
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PubMed id:
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Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.
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F.Jiang,
N.Li,
X.Wang,
J.Cheng,
Y.Huang,
Y.Yang,
J.Yang,
B.Cai,
Y.P.Wang,
Q.Jin,
N.Gao.
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ABSTRACT
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Contractile injection systems (CISs) are cell-puncturing nanodevices that share
ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette
(PVC) represents one group of extracellular CISs that are present in both
bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC
from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage
tail, containing a hexagonal baseplate complex with six fibers and a capped
117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence
of three variants for both tube and sheath proteins, indicating a functional
specialization of them during evolution. The terminal hexameric cap docks onto
the topmost layer of the inner tube and locks the outer sheath in
pre-contraction state with six stretching arms. Our results on the PVC provide a
framework for understanding the general mechanism of widespread CISs and pave
the way for using them as delivery tools in biological or therapeutic
applications.
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Links
