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PDBsum entry 6ii2
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Contents |
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614 a.a.
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568 a.a.
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539 a.a.
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References listed in PDB file
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Key reference
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Title
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Makes caterpillars floppy-Like effector-Containing martx toxins require host ADP-Ribosylation factor (arf) proteins for systemic pathogenicity.
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Authors
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Y.Lee,
B.S.Kim,
S.Choi,
E.Y.Lee,
S.Park,
J.Hwang,
Y.Kwon,
J.Hyun,
C.Lee,
J.F.Kim,
S.H.Eom,
M.H.Kim.
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Ref.
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Proc Natl Acad Sci U S A, 2019,
116,
18031-18040.
[DOI no: ]
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PubMed id
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Abstract
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Upon invading target cells, multifunctional autoprocessing repeats-in-toxin
(MARTX) toxins secreted by bacterial pathogens release their disease-related
modularly structured effector domains. However, it is unclear how a diverse
repertoire of effector domains within these toxins are processed and activated.
Here, we report that Makes caterpillars floppy-like effector (MCF)-containing
MARTX toxins require ubiquitous ADP-ribosylation factor (ARF) proteins for
processing and activation of intermediate effector modules, which localize in
different subcellular compartments following limited processing of holo effector
modules by the internal cysteine protease. Effector domains structured tandemly
with MCF in intermediate modules become disengaged and fully activated by MCF,
which aggressively interacts with ARF proteins present at the same location as
intermediate modules and is converted allosterically into a catalytically
competent protease. MCF-mediated effector processing leads ultimately to severe
virulence in mice via an MCF-mediated ARF switching mechanism across subcellular
compartments. This work provides insight into how bacteria take advantage of
host systems to induce systemic pathogenicity.
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