PDBsum entry 6ef3

Motor protein

PDB id: 6ef3

Contents

Protein chains

196 a.a.

224 a.a.

204 a.a.

195 a.a.

212 a.a.

220 a.a.

208 a.a.

247 a.a.

250 a.a.

242 a.a.

241 a.a.

249 a.a.

234 a.a.

246 a.a.

380 a.a.

384 a.a.

383 a.a.

370 a.a.

360 a.a.

371 a.a.

15 a.a.

280 a.a.

38 a.a.

75 a.a.

Ligands

ATP ×4

ADP ×2

PDB id:

6ef3

Name:

Motor protein

Title:

Yeast 26s proteasome bound to ubiquitinated substrate (4d motor state)

Structure:

Proteasome subunit beta type-1. Chain: 1. Synonym: macropain subunit pre3,multicatalytic endopeptidase complex subunit pre3,proteasome component pre3,proteinase ysce subunit pre3. Engineered: yes. Proteasome subunit beta type-2. Chain: 2. Synonym: macropain subunit pup1,multicatalytic endopeptidase complex subunit pup1,proteasome component pup1,proteinase ysce subunit pup1.

Source:

Saccharomyces cerevisiae s288c. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: pre3, yjl001w, j1407. Expressed in: saccharomyces cerevisiae w303. Expression_system_taxid: 580240. Gene: pup1, yor157c. Gene: pup3, yer094c.

Authors:

A.H.De La Pena,E.A.Goodall,S.N.Gates,G.C.Lander,A.Martin

Key ref:

A.H.De la pena et al. Substrate-Engaged 26 proteasome structures reveal mec for ATP-Hydrolysis-Driven translocation. Science, . PubMed id: 30309908

Date:

15-Aug-18 Release date: 17-Oct-18

Protein chain

P38624  (PSB1_YEAST) -  Proteasome subunit beta type-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 215 a.a.

Struc: 196 a.a.

Protein chain

P25043  (PSB2_YEAST) -  Proteasome subunit beta type-2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 261 a.a.

Struc: 224 a.a.

Protein chain

P25451  (PSB3_YEAST) -  Proteasome subunit beta type-3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 205 a.a.

Struc: 204 a.a.

Protein chain

P22141  (PSB4_YEAST) -  Proteasome subunit beta type-4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 198 a.a.

Struc: 195 a.a.

Protein chain

P30656  (PSB5_YEAST) -  Proteasome subunit beta type-5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 287 a.a.

Struc: 212 a.a.

Protein chain

P23724  (PSB6_YEAST) -  Proteasome subunit beta type-6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 241 a.a.

Struc: 220 a.a.

Protein chain

P30657  (PSB7_YEAST) -  Proteasome subunit beta type-7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 266 a.a.

Struc: 208 a.a.

Protein chain

P21243  (PSA1_YEAST) -  Proteasome subunit alpha type-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 252 a.a.

Struc: 247 a.a.

Protein chain

P23639  (PSA2_YEAST) -  Proteasome subunit alpha type-2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 250 a.a.

Struc: 250 a.a.

Protein chain

P23638  (PSA3_YEAST) -  Proteasome subunit alpha type-3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 258 a.a.

Struc: 242 a.a.

Protein chain

P40303  (PSA4_YEAST) -  Proteasome subunit alpha type-4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 254 a.a.

Struc: 241 a.a.

Protein chain

P32379  (PSA5_YEAST) -  Proteasome subunit alpha type-5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 260 a.a.

Struc: 249 a.a.

Protein chain

P40302  (PSA6_YEAST) -  Proteasome subunit alpha type-6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 234 a.a.

Struc: 234 a.a.

Protein chain

P21242  (PSA7_YEAST) -  Probable proteasome subunit alpha type-7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 288 a.a.

Struc: 246 a.a.

Protein chain

P33299  (PRS7_YEAST) -  26S proteasome regulatory subunit 7 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 467 a.a.

Struc: 380 a.a.

Protein chain

P40327  (PRS4_YEAST) -  26S proteasome regulatory subunit 4 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 437 a.a.

Struc: 384 a.a.

Protein chain

Q01939  (PRS8_YEAST) -  26S proteasome regulatory subunit 8 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 405 a.a.

Struc: 383 a.a.

Protein chain

P33298  (PRS6B_YEAST) -  26S proteasome regulatory subunit 6B homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 428 a.a.

Struc: 370 a.a.

Protein chain

P53549  (PRS10_YEAST) -  26S proteasome subunit RPT4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 437 a.a.

Struc: 360 a.a.

Protein chain

P33297  (PRS6A_YEAST) -  26S proteasome regulatory subunit 6A from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 434 a.a.

Struc: 371 a.a.

Protein chain

P30657  (PSB7_YEAST) -  Proteasome subunit beta type-7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 266 a.a.

Struc: 15 a.a.

Protein chain

P43588  (RPN11_YEAST) -  Ubiquitin carboxyl-terminal hydrolase RPN11 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 306 a.a.

Struc: 280 a.a.

Protein chain

P07818  (CCNB_ARBPU) -  G2/mitotic-specific cyclin-B from Arbacia punctulata

Seq: 409 a.a.

Struc: 38 a.a.*

Protein chain

P0CH08  (RL40A_YEAST) -  Ubiquitin-ribosomal protein eL40A fusion protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 128 a.a.

Struc: 75 a.a.

* PDB and UniProt seqs differ at 15 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains 1, 2, 3, 4, 5, 6, 7, A, B, C, D, E, F, G: E.C.3.4.25.1 - proteasome endopeptidase complex.
• Reaction: Cleavage at peptide bonds with very broad specificity.
• Enzyme class 3: Chain r: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.