PDBsum entry 6ef2

Motor protein

PDB id: 6ef2

Contents

Protein chains

238 a.a.

249 a.a.

241 a.a.

241 a.a.

247 a.a.

232 a.a.

246 a.a.

265 a.a.

260 a.a.

262 a.a.

259 a.a.

264 a.a.

270 a.a.

16 a.a.

Ligands

ATP ×4

ADP ×2

PDB id:

6ef2

Name:

Motor protein

Title:

Yeast 26s proteasome bound to ubiquitinated substrate (5t motor state)

Structure:

Proteasome subunit alpha type-1. Chain: a. Synonym: macropain subunit c7-alpha,multicatalytic endopeptidase complex c7,proteasome component c7-alpha,proteasome component y8, proteinase ysce subunit 7,scl1 suppressor protein. Engineered: yes. Proteasome subunit alpha type-2. Chain: b. Synonym: macropain subunit y7,multicatalytic endopeptidase complex

Source:

Saccharomyces cerevisiae (strain atcc 204508 / s288c). Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: scl1, prc2, prs2, ygl011c. Expressed in: saccharomyces cerevisiae s288c. Expression_system_taxid: 559292. Gene: pre8, prs4, yml092c.

Authors:

A.H.De La Pena,E.A.Goodall,S.N.Gates,G.C.Lander,A.Martin

Key ref:

A.H.De la pena et al. Substrate-Engaged 26 proteasome structures reveal mec for ATP-Hydrolysis-Driven translocation. Science, . PubMed id: 30309908

Date:

15-Aug-18 Release date: 17-Oct-18

Protein chain

P21243  (PSA1_YEAST) -  Proteasome subunit alpha type-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 252 a.a.

Struc: 238 a.a.

Protein chain

P23639  (PSA2_YEAST) -  Proteasome subunit alpha type-2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 250 a.a.

Struc: 249 a.a.

Protein chain

P23638  (PSA3_YEAST) -  Proteasome subunit alpha type-3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 258 a.a.

Struc: 241 a.a.

Protein chain

P40303  (PSA4_YEAST) -  Proteasome subunit alpha type-4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 254 a.a.

Struc: 241 a.a.

Protein chain

P32379  (PSA5_YEAST) -  Proteasome subunit alpha type-5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 260 a.a.

Struc: 247 a.a.

Protein chain

P40302  (PSA6_YEAST) -  Proteasome subunit alpha type-6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 234 a.a.

Struc: 232 a.a.

Protein chain

P21242  (PSA7_YEAST) -  Probable proteasome subunit alpha type-7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 288 a.a.

Struc: 246 a.a.

Protein chain

P33299  (PRS7_YEAST) -  26S proteasome regulatory subunit 7 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 467 a.a.

Struc: 265 a.a.

Protein chain

P40327  (PRS4_YEAST) -  26S proteasome regulatory subunit 4 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 437 a.a.

Struc: 260 a.a.

Protein chain

Q01939  (PRS8_YEAST) -  26S proteasome regulatory subunit 8 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 405 a.a.

Struc: 262 a.a.

Protein chain

P33298  (PRS6B_YEAST) -  26S proteasome regulatory subunit 6B homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 428 a.a.

Struc: 259 a.a.

Protein chain

P53549  (PRS10_YEAST) -  26S proteasome subunit RPT4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 437 a.a.

Struc: 264 a.a.

Protein chain

P33297  (PRS6A_YEAST) -  26S proteasome regulatory subunit 6A from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 434 a.a.

Struc: 270 a.a.

Protein chain

P07818  (CCNB_ARBPU) -  G2/mitotic-specific cyclin-B from Arbacia punctulata

Seq: 409 a.a.

Struc: 16 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G: E.C.3.4.25.1 - proteasome endopeptidase complex.
• Reaction: Cleavage at peptide bonds with very broad specificity.