PDBsum entry 5yqb

Hydrolase

PDB id: 5yqb

Contents

Protein chain

866 a.a.

Ligands

0A1

GMC

GOL

Metals

_ZN

Waters ×869

PDB id:

5yqb

Name:

Hydrolase

Title:

Crystal structure of e.Coli aminopeptidase n in complex with puromycin

Structure:

Aminopeptidase n. Chain: a. Synonym: alpha-aminoacylpeptide hydrolase. Engineered: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: pepn, b0932, jw0915. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.56Å R-factor: 0.162 R-free: 0.182

Authors:

A.K.Marapaka,R.J.Ganji,R.Reddi,A.Addlagatta

Key ref:

A.K.Marapaka et al. Crystal structure of e.Coli aminopeptidase n in compl puromycin. To be published, .

Date:

06-Nov-17 Release date: 14-Nov-18

Protein chain

P04825  (AMPN_ECOLI) -  Aminopeptidase N from Escherichia coli (strain K12)

Seq: 870 a.a.

Struc: 866 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.11.2 - membrane alanyl aminopeptidase.
• Reaction: Release of an N-terminal amino acid, Xaa-|-Xbb- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
• Cofactor: Zn(2+)