PDBsum entry 5lrs

Transcription

PDB id: 5lrs

Contents

Protein chains

236 a.a.

DNA/RNA

Ligands

GSH ×2

Waters ×15

PDB id:

5lrs

Name:

Transcription

Title:

The transcriptional regulator prfa from listeria monocytogenes in complex with glutathione and a 30-bp operator prfa-box motif

Structure:

Listeriolysin positive regulatory factor a. Chain: a, b. Synonym: listeriolysin positive regulatory protein,listeriolysin regulatory protein,pleitrophic regulatory factor a,positive regulatory factor a,prfa. Engineered: yes. DNA (30-mer). Chain: c. Engineered: yes.

Source:

Listeria monocytogenes. Organism_taxid: 1639. Gene: prfa, m643_11230. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 1639

Resolution:

2.90Å R-factor: 0.250 R-free: 0.279

Authors:

M.Hall,C.Grundstrom,A.Begum,M.Lindberg,U.H.Sauer,F.Almqvist, J.Johansson,A.E.Sauer-Eriksson

Key ref:

M.Hall et al. (2016). Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria. Proc Natl Acad Sci U S A, 113, 14733-14738. PubMed id: 27930316 DOI: 10.1073/pnas.1614028114

Date:

19-Aug-16 Release date: 07-Dec-16

Protein chains

P22262  (PRFA_LISMO) -  Listeriolysin regulatory protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

Seq: 237 a.a.

Struc: 236 a.a.

DNA/RNA chains

T-T-G-A-G-G-C-A-T-T-A-A-C-A-T-T-T-G-T-T-A-A-C-G-A-C-G-A-T-A 30 bases

T-A-T-C-G-T-C-G-T-T-A-A-C-A-A-A-T-G-T-T-A-A-T-G-C-C-T-C-A-A 30 bases

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1073/pnas.1614028114

Proc Natl Acad Sci U S A 113:14733-14738 (2016)

PubMed id: 27930316

Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.

M.Hall, C.Grundström, A.Begum, M.J.Lindberg, U.H.Sauer, F.Almqvist, J.Johansson, A.E.Sauer-Eriksson.

ABSTRACT

No abstract given.