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PDBsum entry 5fm7
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Atp binding protein
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PDB id
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5fm7
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References listed in PDB file
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Key reference
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Title
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The combination of X-Ray crystallography and cryo-Electron microscopy provides insight into the overall architecture of the dodecameric rvb1/rvb2 complex.
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Authors
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N.Silva-Martin,
M.I.Daudén,
S.Glatt,
N.A.Hoffmann,
P.Kastritis,
P.Bork,
M.Beck,
C.W.Müller.
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Ref.
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Plos One, 2016,
11,
e0146457.
[DOI no: ]
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PubMed id
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Abstract
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The Rvb1/Rvb2 complex is an essential component of many cellular pathways. The
Rvb1/Rvb2 complex forms a dodecameric assembly where six copies of each subunit
form two heterohexameric rings. However, due to conformational variability, the
way the two rings pack together is still not fully understood. Here, we present
the crystal structure and two cryo-electron microscopy reconstructions of the
dodecameric, full-length Rvb1/Rvb2 complex, all showing that the interaction
between the two heterohexameric rings is mediated through the Rvb1/Rvb2-specific
domain II. Two conformations of the Rvb1/Rvb2 dodecamer are present in solution:
a stretched conformation also present in the crystal, and a compact
conformation. Novel asymmetric features observed in the reconstruction of the
compact conformation provide additional insight into the plasticity of the
Rvb1/Rvb2 complex.
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