PDBsum entry 5ffl

Immune system

PDB id: 5ffl

Contents

Protein chain

113 a.a.

Ligands

EPE

MPD ×2

Metals

_NA

Waters ×112

PDB id:

5ffl

Name:

Immune system

Title:

Crystal structure of mouse cd300lf at 1.6 angstroms resolution.

Structure:

Cd300 antigen-like family member f. Chain: a. Fragment: rankl residues 156-316. Engineered: yes

Source:

Mus musculus. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.60Å R-factor: 0.163 R-free: 0.196

Authors:

C.A.Nelson,D.H.Fremont

Key ref:

R.C.Orchard et al. (2016). Discovery of a proteinaceous cellular receptor for a norovirus. Science, 353, 933-936. PubMed id: 27540007

Date:

18-Dec-15 Release date: 31-Aug-16

Protein chain

Q6SJQ7  (CLM1_MOUSE) -  CMRF35-like molecule 1 from Mus musculus

Seq: 337 a.a.

Struc: 113 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Science 353:933-936 (2016)

PubMed id: 27540007

Discovery of a proteinaceous cellular receptor for a norovirus.

R.C.Orchard, C.B.Wilen, J.G.Doench, M.T.Baldridge, B.T.McCune, Y.C.Lee, S.Lee, S.M.Pruett-Miller, C.A.Nelson, D.H.Fremont, H.W.Virgin.

ABSTRACT

Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host molecules that are essential for murine NoV (MNoV)-induced cell death, including CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV binding and replication in cell lines and primary cells. Additionally, Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in human cells breaks the species barrier that would otherwise restrict MNoV replication. The crystal structure of the CD300lf ectodomain reveals a potential ligand-binding cleft composed of residues that are critical for MNoV infection. Therefore, the presence of a proteinaceous receptor is the primary determinant of MNoV species tropism, whereas other components of cellular machinery required for NoV replication are conserved between humans and mice.