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PDBsum entry 5ffl
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Immune system
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PDB id
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5ffl
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References listed in PDB file
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Key reference
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Title
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Discovery of a proteinaceous cellular receptor for a norovirus.
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Authors
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R.C.Orchard,
C.B.Wilen,
J.G.Doench,
M.T.Baldridge,
B.T.Mccune,
Y.C.Lee,
S.Lee,
S.M.Pruett-Miller,
C.A.Nelson,
D.H.Fremont,
H.W.Virgin.
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Ref.
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Science, 2016,
353,
933-936.
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PubMed id
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Abstract
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Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host
factors required for NoV infection are poorly understood. We identified host
molecules that are essential for murine NoV (MNoV)-induced cell death, including
CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV
binding and replication in cell lines and primary cells. Additionally,
Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in
human cells breaks the species barrier that would otherwise restrict MNoV
replication. The crystal structure of the CD300lf ectodomain reveals a potential
ligand-binding cleft composed of residues that are critical for MNoV infection.
Therefore, the presence of a proteinaceous receptor is the primary determinant
of MNoV species tropism, whereas other components of cellular machinery required
for NoV replication are conserved between humans and mice.
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