PDBsum entry 5d7l

Immune system

PDB id: 5d7l

Contents

Protein chains

262 a.a.

98 a.a.

242 a.a.

187 a.a.

241 a.a.

189 a.a.

Ligands

2LJ ×2

References listed in PDB file

Key reference

• Title: Diversity of t cells restricted by the mhc class i-Related molecule mr1 facilitates differential antigen recognition.
• Authors: N.A.Gherardin, A.N.Keller, R.E.Woolley, J.Le nours, D.S.Ritchie, P.J.Neeson, R.W.Birkinshaw, S.B.Eckle, J.N.Waddington, L.Liu, D.P.Fairlie, A.P.Uldrich, D.G.Pellicci, J.Mccluskey, D.I.Godfrey, J.Rossjohn.
• Ref.: Immunity, 2016, 44, 32-45. [DOI no: 10.1016/j.immuni.2015.12.005]
• PubMed id: 26795251

Abstract

A characteristic of mucosal-associated invariant T (MAIT) cells is the expression of TRAV1-2(+) T cell receptors (TCRs) that are activated by riboflavin metabolite-based antigens (Ag) presented by the MHC-I related molecule, MR1. Whether the MR1-restricted T cell repertoire and associated Ag responsiveness extends beyond these cells remains unclear. Here, we describe MR1 autoreactivity and folate-derivative reactivity in a discrete subset of TRAV1-2(+) MAIT cells. This recognition was attributable to CDR3β loop-mediated effects within a consensus TRAV1-2(+) TCR-MR1-Ag footprint. Furthermore, we have demonstrated differential folate- and riboflavin-derivative reactivity by a diverse population of "atypical" TRAV1-2(-) MR1-restricted T cells. We have shown that TRAV1-2(-) T cells are phenotypically heterogeneous and largely distinct from TRAV1-2(+) MAIT cells. A TRAV1-2(-) TCR docks more centrally on MR1, thereby adopting a markedly different molecular footprint to the TRAV1-2(+) TCR. Accordingly, diversity within the MR1-restricted T cell repertoire leads to differing MR1-restricted Ag specificity.