PDBsum entry 5zlz

Hydrolase inhibitor/hydrolase

PDB id: 5zlz

Contents

Protein chains

372 a.a.

248 a.a.

Ligands

GOL ×2

PDB id:

5zlz

Name:

Hydrolase inhibitor/hydrolase

Title:

Structure of tpa and pai-1

Structure:

Plasminogen activator inhibitor 1. Chain: i. Synonym: pai-1,endothelial plasminogen activator inhibitor,serpin e1. Engineered: yes. Mutation: yes. Tissue-type plasminogen activator. Chain: e. Synonym: tpa. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: serpine1, pai1, planh1. Expressed in: prokaryotic environmental samples. Expression_system_taxid: 81490. Gene: plat. Expression_system_taxid: 81490

Resolution:

3.58Å R-factor: 0.228 R-free: 0.312

Authors:

L.Min,M.Huang

Key ref:

L.Min and m.huang Development of a pai-1 trapping agent (paitrap2) base inactivated tpa-Spd and the crystal structure of pait complex with pai-1. To be published, .

Date:

31-Mar-18 Release date: 03-Apr-19

Protein chain

P05121  (PAI1_HUMAN) -  Plasminogen activator inhibitor 1 from Homo sapiens

Seq: 402 a.a.

Struc: 372 a.a.*

Protein chain

P00750  (TPA_HUMAN) -  Tissue-type plasminogen activator from Homo sapiens

Seq: 562 a.a.

Struc: 248 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.68 - t-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.