PDBsum entry 5j4s

Hydrolase

PDB id: 5j4s

Contents

Protein chains

241 a.a.

67 a.a.

Waters ×206

PDB id:

5j4s

Name:

Hydrolase

Title:

Alpha-chymotrypsin from bovine pancreas in complex with a modified bowman-birk inhibitor from soybean

Structure:

Chymotrypsinogen a. Chain: a. Other_details: sigma-aldrich catalogue id c4129. Bowman-birk type proteinase inhibitor. Chain: b. Fragment: unp residues 40-110. Synonym: bbi. Engineered: yes. Mutation: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Synthetic: yes. Glycine max. Soybean. Organism_taxid: 3847

Resolution:

2.10Å R-factor: 0.193 R-free: 0.241

Authors:

E.Johansson,C.W.Tornoee

Key ref:

C.W.Tornoee et al. Divergent protein synthesis of bowman-Birk inhibitor analogues by native chemical ligation. To be published, .

Date:

01-Apr-16 Release date: 12-Apr-17

Protein chain

P00766  (CTRA_BOVIN) -  Chymotrypsinogen A from Bos taurus

Seq: 245 a.a.

Struc: 241 a.a.

Protein chain

P01055  (IBB1_SOYBN) -  Bowman-Birk type proteinase inhibitor from Glycine max

Seq: 110 a.a.

Struc: 67 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.21.1 - chymotrypsin.
• Reaction: Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.