PDBsum entry 4wnh

Transferase/protein binding

PDB id: 4wnh

Contents

Protein chains

298 a.a.

35 a.a.

Ligands

BGC-XYS

UDX

Metals

_MN

Waters ×88

PDB id:

4wnh

Name:

Transferase/protein binding

Title:

Crystal structure of mouse xyloside xylosyltransferase 1 complexed with manganese,acceptor ligand and udp-xylose

Structure:

Xyloside xylosyltransferase 1. Chain: a. Fragment: unp residues 87-392. Synonym: udp-xylose:alpha-xyloside alpha-1,3-xylosyltransferase. Engineered: yes. Coagulation factor ix. Chain: d. Fragment: unp residues 92-130. Synonym: christmas factor,plasma thromboplastin component,ptc.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: xxylt1. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293t. Homo sapiens. Human.

Resolution:

1.95Å R-factor: 0.194 R-free: 0.224

Authors:

H.Yu,H.Li

Key ref:

H.Yu and h.li Crystal structure of mouse xyloside xylosyltransferas complexed with manganese,Acceptor ligand and udp-Xylo. To be published, .

Date:

11-Oct-14 Release date: 30-Sep-15

Protein chain

Q3U4G3  (XXLT1_MOUSE) -  Xyloside xylosyltransferase 1 from Mus musculus

Seq: 392 a.a.

Struc: 298 a.a.

Protein chain

P00740  (FA9_HUMAN) -  Coagulation factor IX from Homo sapiens

Seq: 461 a.a.

Struc: 35 a.a.

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.4.2.62 - xylosyl alpha-1,3-xylosyltransferase.
• Reaction: 3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1->3)-alpha-D- xylosyl-(1->3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H⁺

3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose (Bound ligand (Het Group name = UDX) corresponds exactly) = 3-O-[alpha-D-xylosyl-(1->3)-alpha-D- xylosyl-(1->3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)

• Enzyme class 2: Chain D: E.C.3.4.21.22 - coagulation factor IXa.
• Reaction: Hydrolyzes one Arg-|-Ile bond in factor X to form factor Xa.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site