PDBsum entry 4trt

Transferase

PDB id: 4trt

Contents

Protein chains

361 a.a.

Waters ×292

PDB id:

4trt

Name:

Transferase

Title:

Deinococcus radiodurans DNA polymerase iii subunit beta

Structure:

DNA polymerase iii subunit beta. Chain: a, b. Engineered: yes. Other_details: the last seven residues are not visible in the electron density. The last six residues are cloning artefacts. The uniprot entry q9rye8 sequence is incorrect due to an annotation mistake in the genomic sequence leading to a frameshift. This is corrected in our sequence giving a protein of 362 aa.

Source:

Deinococcus radiodurans. Organism_taxid: 243230. Strain: atcc 13939 / dsm 20539 / jcm 16871 / lmg 4051 / nbrc 15346 / ncimb 9279 / r1 / vkm b-1422. Gene: dr_0001. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.200 R-free: 0.235

Authors:

L.Niiranen,K.Lian,K.A.Johnson,E.Moe

Key ref:

L.Niiranen et al. (2015). Crystal structure of the DNA polymerase III β subunit (β-clamp) from the extremophile Deinococcus radiodurans. Bmc Struct Biol, 15, 5. PubMed id: 25886944 DOI: 10.1186/s12900-015-0032-6

Date:

17-Jun-14 Release date: 29-Apr-15

Protein chains

Q9RYE8  (Q9RYE8_DEIRA) -  Beta sliding clamp from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)

Seq: 393 a.a.

Struc: 361 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1186/s12900-015-0032-6

Bmc Struct Biol 15:5 (2015)

PubMed id: 25886944

Crystal structure of the DNA polymerase III β subunit (β-clamp) from the extremophile Deinococcus radiodurans.

L.Niiranen, K.Lian, K.A.Johnson, E.Moe.

ABSTRACT

No abstract given.