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PDBsum entry 4ruc
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Transferase/DNA
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PDB id
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4ruc
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References listed in PDB file
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Key reference
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Title
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Structural basis for error-Free bypass of the 5-N-Methylformamidopyrimidine-Dg lesion by human DNA polymerase η and sulfolobus solfataricus p2 polymerase IV.
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Authors
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A.Patra,
S.Banerjee,
T.L.Johnson salyard,
C.K.Malik,
P.P.Christov,
C.J.Rizzo,
M.P.Stone,
M.Egli.
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Ref.
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J Am Chem Soc, 2015,
137,
7011-7014.
[DOI no: ]
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PubMed id
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Abstract
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N(6)-(2-Deoxy-d-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine
(MeFapy-dG) arises from N7-methylation of deoxyguanosine followed by imidazole
ring opening. The lesion has been reported to persist in animal tissues.
Previous in vitro replication bypass investigations of the MeFapy-dG adduct
revealed predominant insertion of C opposite the lesion, dependent on the
identity of the DNA polymerase (Pol) and the local sequence context. Here we
report crystal structures of ternary Pol·DNA·dNTP complexes between
MeFapy-dG-adducted DNA template:primer duplexes and the Y-family polymerases
human Pol η and P2 Pol IV (Dpo4) from Sulfolobus solfataricus. The structures
of the hPol η and Dpo4 complexes at the insertion and extension stages,
respectively, are representative of error-free replication, with MeFapy-dG in
the anti conformation and forming Watson-Crick pairs with dCTP or dC.
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