PDBsum entry 4pha

Transferase,lyase/DNA

PDB id: 4pha

Contents

Protein chain

327 a.a.

DNA/RNA

Ligands

0KX

Metals

_MG ×2

_NA ×2

Waters ×74

References listed in PDB file

Key reference

• Title: The spontaneous replication error and the mismatch discrimination mechanisms of human DNA polymerase β.
• Authors: M.C.Koag, K.Nam, S.Lee.
• Ref.: Nucleic Acids Res, 2014, 42, 11233-11245. [DOI no: 10.1093/nar/gku789]
• PubMed id: 25200079

Abstract

To provide molecular-level insights into the spontaneous replication error and the mismatch discrimination mechanisms of human DNA polymerase β (polβ), we report four crystal structures of polβ complexed with dG•dTTP and dA•dCTP mismatches in the presence of Mg(2+) or Mn(2+). The Mg(2+)-bound ground-state structures show that the dA•dCTP-Mg(2+) complex adopts an 'intermediate' protein conformation while the dG•dTTP-Mg(2+) complex adopts an open protein conformation. The Mn(2+)-bound 'pre-chemistry-state' structures show that the dA•dCTP-Mn(2+) complex is structurally very similar to the dA•dCTP-Mg(2+) complex, whereas the dG•dTTP-Mn(2+) complex undergoes a large-scale conformational change to adopt a Watson-Crick-like dG•dTTP base pair and a closed protein conformation. These structural differences, together with our molecular dynamics simulation studies, suggest that polβ increases replication fidelity via a two-stage mismatch discrimination mechanism, where one is in the ground state and the other in the closed conformation state. In the closed conformation state, polβ appears to allow only a Watson-Crick-like conformation for purine•pyrimidine base pairs, thereby discriminating the mismatched base pairs based on their ability to form the Watson-Crick-like conformation. Overall, the present studies provide new insights into the spontaneous replication error and the replication fidelity mechanisms of polβ.