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PDBsum entry 4nsx
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Protein binding
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PDB id
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4nsx
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References listed in PDB file
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Key reference
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Title
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Structure of utp21 tandem wd domain provides insight into the organization of the utpb complex involved in ribosome synthesis.
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Authors
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C.Zhang,
J.Lin,
W.Liu,
X.Chen,
R.Chen,
K.Ye.
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Ref.
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Plos One, 2014,
9,
e86540.
[DOI no: ]
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PubMed id
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Abstract
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Assembly of the eukaryotic ribosome requires a large number of trans-acting
proteins and small nucleolar RNAs that transiently associate with the precursor
rRNA to facilitate its modification, processing and binding with ribosomal
proteins. UTPB is a large evolutionarily conserved complex in the 90S small
subunit processome that mediates early processing of 18S rRNA. UTPB consists of
six proteins Utp1/Pwp1, Utp6, Utp12/Dip2, Utp13, Utp18 and Utp21 and has
abundant WD domains. Here, we determined the crystal structure of the tandem WD
domain of yeast Utp21 at 2.1 Å resolution, revealing two open-clamshell-shaped
β-propellers. The bottom faces of both WD domains harbor several conserved
patches that potentially function as molecular binding sites. We show that
residues 100-190 of Utp18 bind to the tandem WD domain of Utp21. Structural
mapping of previous crosslinking data shows that the WD domains of Utp18 and
Utp1 are organized on two opposite sides of the Utp21 WD domains. This study
reports the first structure of a UTPB component and provides insight into the
structural organization of the UTPB complex.
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