PDBsum entry 4nsx

Protein binding

PDB id: 4nsx

Contents

Protein chain

634 a.a.

Ligands

FLC

Waters ×394

PDB id:

4nsx

Name:

Protein binding

Title:

Crystal structure of the utp21 tandem wd domain

Structure:

U3 small nucleolar RNA-associated protein 21. Chain: a. Fragment: tandem wd domain 1-684. Synonym: u3 snorna-associated protein 21, u three protein 21. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: l8084.22, utp21, ylr409c. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.189 R-free: 0.224

Authors:

C.Zhang,K.Ye

Key ref:

C.Zhang et al. (2014). Structure of Utp21 tandem WD domain provides insight into the organization of the UTPB complex involved in ribosome synthesis. Plos One, 9, e86540. PubMed id: 24466140 DOI: 10.1371/journal.pone.0086540

Date:

29-Nov-13 Release date: 19-Feb-14

Protein chain

Q06078  (UTP21_YEAST) -  U3 small nucleolar RNA-associated protein 21 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 939 a.a.

Struc: 634 a.a.

DOI no: 10.1371/journal.pone.0086540

Plos One 9:e86540 (2014)

PubMed id: 24466140

Structure of Utp21 tandem WD domain provides insight into the organization of the UTPB complex involved in ribosome synthesis.

C.Zhang, J.Lin, W.Liu, X.Chen, R.Chen, K.Ye.

ABSTRACT

Assembly of the eukaryotic ribosome requires a large number of trans-acting proteins and small nucleolar RNAs that transiently associate with the precursor rRNA to facilitate its modification, processing and binding with ribosomal proteins. UTPB is a large evolutionarily conserved complex in the 90S small subunit processome that mediates early processing of 18S rRNA. UTPB consists of six proteins Utp1/Pwp1, Utp6, Utp12/Dip2, Utp13, Utp18 and Utp21 and has abundant WD domains. Here, we determined the crystal structure of the tandem WD domain of yeast Utp21 at 2.1 Å resolution, revealing two open-clamshell-shaped β-propellers. The bottom faces of both WD domains harbor several conserved patches that potentially function as molecular binding sites. We show that residues 100-190 of Utp18 bind to the tandem WD domain of Utp21. Structural mapping of previous crosslinking data shows that the WD domains of Utp18 and Utp1 are organized on two opposite sides of the Utp21 WD domains. This study reports the first structure of a UTPB component and provides insight into the structural organization of the UTPB complex.