PDBsum entry 4mzy

Transferase

PDB id: 4mzy

Contents

Protein chain

486 a.a.

Ligands

PO4 ×2

GOL ×12

MLA ×3

FMT

Metals

_CL ×4

Waters ×171

PDB id:

4mzy

Name:

Transferase

Title:

Crystal structure of enterococcus faecalis nicotinate phosphoribosyltransferase with malonate and phosphate bound

Structure:

Nicotinate phosphoribosyltransferase. Chain: a. Engineered: yes

Source:

Enterococcus faecalis. Organism_taxid: 226185. Strain: v583. Gene: ef2626. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.95Å R-factor: 0.155 R-free: 0.186

Authors:

Y.Patskovsky,R.Toro,S.R.Wasserman,S.C.Almo,S.K.Burley,New York Sgx Research Center For Structural Genomics (Nysgxrc),New York Structural Genomics Research Consortium (Nysgrc)

Key ref:

Y.Patskovsky et al. Crystal structure of enterococcus faecalis nicotinate phosphoribosyltransferase. To be published, .

Date:

30-Sep-13 Release date: 23-Oct-13

Protein chain

Q830Y8  (Q830Y8_ENTFA) -  Nicotinate phosphoribosyltransferase from Enterococcus faecalis (strain ATCC 700802 / V583)

Seq: 494 a.a.

Struc: 486 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.3.4.21 - nicotinate phosphoribosyltransferase.
• Reaction: nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate

nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O (Bound ligand (Het Group name = MLA) matches with 45.45% similarity) = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site