PDBsum entry 4i2o

Transcription regulator/DNA

PDB id: 4i2o

Contents

Protein chains

198 a.a.

DNA/RNA

Waters ×496

PDB id:

4i2o

Name:

Transcription regulator/DNA

Title:

The structure of fixk2 from bradyrhizobium japonicum

Structure:

Fixk2 protein. Chain: a, b. Engineered: yes. Mutation: yes. Promoter of fixk2 direct target, fixn, upstream. Chain: w. Engineered: yes. Promoter of fixk2 direct target, fixn, downstream. Chain: x.

Source:

Bradyrhizobium japonicum. Organism_taxid: 375. Gene: fixk2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

1.77Å R-factor: 0.185 R-free: 0.227

Authors:

M.Bonnet,M.Kurz,S.Mesa,C.Briand,H.Hennecke,M.G.Gruetter

Key ref:

M.Bonnet et al. (2013). The structure of Bradyrhizobium japonicum transcription factor FixK2 unveils sites of DNA binding and oxidation. J Biol Chem, 288, 14238-14246. PubMed id: 23546876 DOI: 10.1074/jbc.M113.465484

Date:

22-Nov-12 Release date: 10-Apr-13

Protein chains

O69245  (O69245_BRAJP) -  FixK2 protein from Bradyrhizobium japonicum

Seq: 232 a.a.

Struc: 198 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-G-G-A-A-T-T-G-A-T-T-G-A-A-A-T-C-A-A-G-A-T-A-G-G 25 bases

C-T-A-T-C-T-T-G-A-T-T-T-C-A-A-T-C-A-A-T-T-C-C-C 24 bases

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1074/jbc.M113.465484

J Biol Chem 288:14238-14246 (2013)

PubMed id: 23546876

The structure of Bradyrhizobium japonicum transcription factor FixK2 unveils sites of DNA binding and oxidation.

M.Bonnet, M.Kurz, S.Mesa, C.Briand, H.Hennecke, M.G.Grütter.

ABSTRACT

No abstract given.