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PDBsum entry 4gl2
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RNA binding protein/RNA
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PDB id
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4gl2
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References listed in PDB file
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Key reference
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Title
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Structural basis for dsrna recognition, Filament formation, And antiviral signal activation by mda5.
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Authors
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B.Wu,
A.Peisley,
C.Richards,
H.Yao,
X.Zeng,
C.Lin,
F.Chu,
T.Walz,
S.Hur.
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Ref.
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Cell, 2013,
152,
276-289.
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PubMed id
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Abstract
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MDA5, a viral double-stranded RNA (dsRNA) receptor, shares sequence similarity
and signaling pathways with RIG-I yet plays essential functions in antiviral
immunity through distinct specificity for viral RNA. Revealing the molecular
basis for the functional divergence, we report here the crystal structure
of MDA5 bound to dsRNA, which shows how, using the same domain architecture,
MDA5 recognizes the internal duplex structure, whereas RIG-I recognizes the
terminus of dsRNA. We further show that MDA5 uses direct protein-protein
contacts to stack along dsRNA in a head-to-tail arrangement, and that the
signaling domain (tandem CARD), which decorates the outside of the core MDA5
filament, also has an intrinsic propensity to oligomerize into an elongated
structure that activates the signaling adaptor, MAVS. These data support a model
in which MDA5 uses long dsRNA as a signaling platform to cooperatively assemble
the core filament, which in turn promotes stochastic assembly of the tandem CARD
oligomers for signaling.
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