PDBsum entry 4d25

Hydrolase

PDB id: 4d25

Contents

Protein chain

427 a.a.

DNA/RNA

Ligands

ANP

GOL

Metals

_MG

Waters ×330

PDB id:

4d25

Name:

Hydrolase

Title:

Crystal structure of the bombyx mori vasa helicase (e339q) in complex with RNA and amppnp

Structure:

Bmvlg protein. Chain: a. Fragment: helicase, residues 135-564. Synonym: bm vasa. Engineered: yes. Mutation: yes. 5'-r( Up Gp Ap Cp Ap Up)-3'. Chain: d. Engineered: yes

Source:

Bombyx mori. Domestic silkworm. Organism_taxid: 7091. Cell_line: bmn4 ovarian cell culture. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: star. Synthetic: yes. Organism_taxid: 7091

Resolution:

1.90Å R-factor: 0.173 R-free: 0.208

Authors:

P.Spinelli,R.S.Pillai,J.Kadlec,S.Cusack

Key ref:

J.Xiol et al. (2014). RNA clamping by Vasa assembles a piRNA amplifier complex on transposon transcripts. Cell, 157, 1698-1711. PubMed id: 24910301 DOI: 10.1016/j.cell.2014.05.018

Date:

07-May-14 Release date: 18-Jun-14

Protein chain

O01378  (O01378_BOMMO) -  RNA helicase from Bombyx mori

Seq: 601 a.a.

Struc: 427 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chain

U-G-A-C-A-U 6 bases

Enzyme reactions

• Enzyme class: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.cell.2014.05.018

Cell 157:1698-1711 (2014)

PubMed id: 24910301

RNA clamping by Vasa assembles a piRNA amplifier complex on transposon transcripts.

J.Xiol, P.Spinelli, M.A.Laussmann, D.Homolka, Z.Yang, E.Cora, Y.Couté, S.Conn, J.Kadlec, R.Sachidanandam, M.Kaksonen, S.Cusack, A.Ephrussi, R.S.Pillai.

ABSTRACT

No abstract given.